云南傣族、汉族HLA-G基因14bp插入/缺失多态性研究

Analysis of the 14 bp insertion/deletion polymorphism in human leukocyte antigen-G gene in Chinese Dai and Han ethnic groups in Yunnan Province

14bp插入/缺失多态性是指存在于HLA-G基因第8外显子3′非翻译区(3′UTR)的一个插入/缺失多态,因其能影响HLA-GmRNA的稳定性,并进一步影响HLA-G蛋白的翻译而受到广泛关注。文章采用PCR和电泳技术对云南傣族和汉族两个人群进行了HLA-G基因14bp插入/缺失多态性的检测分析,结果显示傣族和汉族人群+14bp等位基因频率分别为31.97%和40.87%,+14bp/+14bp基因型频率分别为8.20%和17.31%,+14bp/-14bp基因型频率分别为47.54%和47.11%。与国内外已报道的其他人群的数据比较表明:云南汉族群体中HLA-G基因14bp插入/缺失的分布与其他群体相似;傣族则有自己独特的基因型和等位基因分布特点,推测其可能受到了遗传漂变的作用,但不排除自然选择作用的影响。

A 14 bp insertion/deletion polymorphism （＋14 bp/-14 bp） in exon 8 of HLA-G gene has been investigated extensively because this polymorphism was associated with HLA-G mRNA stability and could influence HLA-G mRNA expression. In the present study, we genotyped 14 bp insertion/deletion polymorphisms in the HLA-G gene in Dai and Han populations in Yunnan Province. The frequencies of ＋14 bp in Dai and Han were 31.97% and 40.87%, respectively. The frequency of ＋14 bp/＋14 bp in Dai was lower （8.20%） than that of Han （17.31%）. Nevertheless, the frequency of ＋14 bp/-14 bp was similar in both populations （47.54% in Dai and 47.11% in Han）. When compared with other populations, the frequency of 14 bp insertion/deletion of Han was similar to others, whereas the Dai showed significent difference from others and presented it’s specific characteristic. We assumed that the distributaion characteristic of 14 bp insertion/deletionin Dai might be the results of random genetic drift, but the role of natural selection cannot be excluded.