不同压强与时程CO_2气腹对胃癌细胞黏附侵袭能力的影响

Influence of Different Pressures and Time of CO_2 Pneumoperitoneum on Adhesive and Invasive Ability of Gastric Cancer Cells

目的分析不同压强与时程的CO2气腹对胃癌细胞黏附侵袭能力的影响。方法建立体外CO2气腹模型,选用3种胃癌细胞株MKN-45、SGC-7901和MKN-28,分别暴露在0mm Hg(1mm Hg=0.133kPa)、9mm Hg(2h、4h)和15mm Hg(2h、4h)的条件下。RT-PCR法检测胃癌细胞中黏附侵袭分子E-cadherin、细胞间黏附分子-1(ICAM-1)、金属基质蛋白酶-2(MMP-2)和血管内皮生长因子-A(VEGF-A)mRNA在上述条件下的表达水平;流式细胞仪检测胃癌细胞中E-cadherin和ICAM-1蛋白在0mm Hg和15mm Hg(4h)条件下的表达水平。结果随着CO2时程延长或压强升高,E-cadherin mRNA的表达水平有下降趋势,而ICAM-1、MMP-2和VEGF-A mRNA的表达水平则有上升趋势;流式细胞仪检测结果显示E-cadherin蛋白的表达水平有下降趋势,而ICAM-1蛋白的表达水平有升高的趋势。但各种条件下黏附侵袭分子的表达水平比较,差异均无统计学意义(P>0.05)。结论在压强不高于15mm Hg和时程不超过4h前提下,不同压强与时程的CO2气腹对胃癌细胞株的黏附侵袭能力无明显影响,并不增加肿瘤的转移几率。

Objective To investigate the influence of different pressures and duration of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expressions of adhesive and invasive molecules.Methods With an artificial CO2 pneumoperitoneum model in vitro,human gastric cancer cell lines including MKN-45,SGC-7901,and MKN-28 were exposed to CO2 in different environments：0 mm Hg（1 mm Hg=0.133 kPa）,9 mm Hg（2 h,4 h）,and 15 mm Hg（2 h,4 h）.The expressions of mRNA of E-cadherin,intercellular adhesion molecule-1（ICAM-1）,matrix metalloproteinase-2（MMP-2）,and vascular endothelial growth factor-A（VEGF-A）in the different environments were measured by RT-PCR.The expressions of protein of E-cadherin and ICAM-1 in the environments of 0 mm Hg and 15 mm Hg（4 h）were measured by FCM.Results With the increase of duration or pressure,RT-PCR showed that there was a downward trend in the expression of E-cadherin mRNA as well as there were upward trends in the expressions of ICAM-1,MMP-2,and VEGF-A mRNA;FCM showed that there was a downward trend in the expression of E-cadherin protein while the expression of ICAM-1 protein showed the opposite change.But there were no obvious differences under different environment（P〉0.05）.Conclusions Under low pressure（≤15 mm Hg）and short time（≤4 h）of CO2 pneumoperitoneum,the adhesive and invasive ability of gastric cancer cells could not be affected,which means that under this environment,CO2 pneumoperitoneum will not increase the possibility of neoplasm metastasis.