7种microRNAs在原发性肝癌组织和癌旁组织间的差异表达及与血清肿瘤标志物水平的相关性研究

Differential Expressions of Seven MicroRNAs Between Primary Hepatocellular Carcinoma and Adjacent Nontumorous Tissues and Their Correlations with Levels of Tumor Markers in Serum

目的分析小RNA(microRNAs)在原发性肝细胞癌组织和癌旁组织中的表达差异及与血清肿瘤标志物水平的相关性,探索肝癌诊断和预后可能的新标志。方法使用实时定量PCR技术检测7种MicroRNAs在原发性肝细胞癌组织和癌旁组织中的表达差异。结果 7种microRNAs在原发性肝细胞癌组织和癌旁组织中的表达差异均有统计学意义(P<0.05)。与癌旁组织相比,mi R-34c、mi R-21、mi R-16及mi R-10b在肝癌组织中表达上调,mi R-200a、mi R-148b及mi R-Let-7i在肝癌组织中表达下调,其中mi R-200a和mi R-148b下调明显。mi R-200a在肝癌和癌旁组织中的表达差异与患者血清AFP水平呈正相关(r=0.8489,P<0.01),而其余microRNAs的表达差异与患者血清各肿瘤标志物水平均无相关关系(P>0.05)。结论原发性肝细胞癌和癌旁组织中存在microRNAs的表达差异,mi R-200a可能成为新的肝癌早期诊断标志。

Objective To explore the differential expressions of seven microRNAs between hepatocellular carcinoma（HCC）and adjacent nontumorous tissues（NT）,analyze the correlations between differential expressing microRNAs and the levels of tumor markers in serum,and furnish evidence for novel diagnostic and prognostic tool of HCC.Methods Real-time quantitative PCR technique was used to measure the differential expressions of seven microRNAs in HCC tissues compared with NT.Results Compared with NT,the relative expressions of seven microRNAs in HCC tissues manifested statistical difference（P〈0.05）.MiR-34c,miR-21,miR-16,and miR-10b presented higher expressions in the HCC samples than those in the NT samples,while miR-200a,miR-148b,and miR-Let-7i demonstrated lower expressions in the HCC samples than those in the NT samples.In addition,miR-200a and miR-148b were markedly down-regulated in the HCC tissues than those in the NT.The differential expressions of miR-200a in HCC compared with NT samples was correlated with serum AFP level of the patients（r=0.848 9,P〈0.01）,while the differential expressions of the other six microRNAs had no correlation with the levels of tumor markers in serum（P〈0.05）.Conclusions There are differential expressions of microRNAs between HCC and NT.MiR-200a may serve as a novel diagnostic and prognostic tool of HCC.