四君子汤对家兔肠梗阻解除后肠黏膜屏障的影响

Effect of Sijunzi(四君子)Decoction on Restoration of Gut Barrier after Relief of Intestinal Obstruction in Rabbits Intestine

目的:观察家兔肠梗阻解除后小肠黏膜屏障恢复情况及四君子汤对其影响。方法:大耳白兔60只,体重2.5～3kg,随机分成6组:假手术组(SO)、可复性肠梗阻模型梗阻组(I48)、梗阻解除后自然恢复组(S48、S96)及四君子汤治疗组(T48、T96),治疗组管饲四君子汤中药煎剂5mL/kg,2次/d,其余各组管饲同量生理盐水,于相应时段分别检测家兔血中D-乳酸、肠脂肪酸结合蛋白及肠液中黏蛋白的含量,观察肠黏膜病理形态变化并行肠黏膜损伤评分。结果:肠梗阻解除后,血D-乳酸水平逐渐降低、肠黏蛋白含量逐渐升高(P<0.05),中药组与自然恢复组相比,血清D-乳酸、肠脂肪酸结合蛋白含量降低,肠黏蛋白含量增加,肠黏膜损伤评分减低,差别有统计学意义(P<0.05)。结论:四君子汤能在肠梗阻解除后减轻肠黏膜损害,促进肠黏膜损伤后修复,具有保护肠黏膜屏障的作用。

Objective To investigate the effect of Sijunzi（四君子）Decoction on the restoration process of gut barrier after the relief of intestinal obstruction in rabbit intestine. Methods Sixty rabbits with body weight of 2.5～3 kg were divided into 6 groups randomLy, including the sham operation group, controllable ileus group, natural recovery group and Chinese herbal medicine treatment group. The concentration of D-lactate and intestinal fatty acid-binding protein in the plasma, intestinal mucin and Chiu’s histopathological evaluation score were detected on the corresponding time levels. Results In the treatment group the level of D-lactate was reduced gradually, while the level of intestinal mucin increased gradually. Compared with the natural recovery groups the levels of D-lactate, IFABP and Chiu’s histopathological evaluation score in the treatment groups reduced obviously, and the level of intestinal mucin increased obviously with statistical significances after the relieve of intestinal obstruction. Conclusion The Chinese traditional herbal medicine Sijunzi Decoction shows to be beneficial in decreasing the damages of intestinal mucosa after the relieve of intestinal obstruction.