蒿甲醚对SD大鼠原位脑胶质瘤的抑瘤实验

Inhibitive Effect of Artemether on Glioma Growth in SD Rat Orthotopic Glioma Model

目的探讨蒿甲醚(Artemether)对SD大鼠原位脑胶质瘤的抑瘤作用。方法采用四甲基偶氮唑蓝(MTT)法测定不同浓度蒿甲醚对大鼠C6脑胶质瘤细胞株的生长抑制作用,计算半数抑制浓度(IC50)。立体定位仪对48只(雌、雄各半)SD大鼠建立脑胶质瘤原位模型,随机分为6组:对照组;模型组,蒿甲醚33.3mg/(kg·d)、50.0mg/(kg·d)、66.6mg/(kg·d);联合用药组,蒿甲醚50.0mg/(kg·d)+硫酸亚铁1.5mg/(kg·d);阳性药对照组。采用灌胃给药法连续给予各组SD大鼠用药10天,对照组给予0.9%氯化钠溶液。肿瘤体积按a2bπз6(a为肿瘤的短径,b为肿瘤的长径)计算。全脑标本用4%多聚甲醛固定。肿瘤组织做病理观察。结果蒿甲醚抑制大鼠C6脑胶质瘤细胞增殖,其抑制作用呈剂量和时间依赖性;模型组和联合用药组对SD大鼠原位脑胶质瘤的抑瘤率分别为:54.5%、61.0%、64.5%和69.8%;模型组组间比较,蒿甲醚66.6mg/(kg·d)用药组的抑瘤率明显高于蒿甲醚33.3mg/(kg·d)用药组;各实验组间比较,联合用药组的抑瘤率显著高于模型组33.3mg/(kg·d)。结论口服蒿甲醚对SD大鼠脑部原位接种C6脑胶质瘤有明显的抑瘤作用。

Objective To explore the mechanisms of inhibition of artemether （Artm） on glioma growth in brain tumor bearing SD rat.Methods MTT （Methy thiazolyl tetrazolium） assay was used to evaluate the inhibitory effect of artemether treatment on C6 glioma cells. Forty-eight （24 male and 24 female） SD rats which were subcutaneous planted with SD rat C6 glioma cell to establish SD rat orthotopic glioma model were divided resourcefully into 6 groups. They were control group; model groups, artemether 33.3 mg/（kg·d）, 50 mg/（kg·d）, 66.6 mg/（kg·d）; two drugs combined group, artm 50 mg/（kg·d）＋ ferrous sulfate 1.5 mg/（kg·d） and positive control group. Lomustine 20 mg/（kg·d） SD rats of each group were fed for 10 consecutive days. Inhibitive rates of Artemether were calculated by a2bπ∕6.Results Artemether could inhibit the proliferation of C6 glioma cells in concentration and time -depended manners. Inhibitive rates of artemether in different dosages were 54.5%、61.0%、64.5% and 69.8% respectively. Compared among model groups, inhibitive rates of artemether 66.6 mg/（kg·d） was higher than that of artemether 33.3 mg/（kg·d）, there was a significant difference between two group （P0.05）. Compared among experiment groups, inhibitive rate of Artm ＋ ferrous sulfate was higher than that of 33.3 mg/（kg·d）, there was a significant difference （P0.05）.Conclusion Artemether can inhibit glioma growth in SD rat orthotopic glioma model.