大肠癌组织中unc5c基因启动子甲基化的分析

Aberrant Methylation of unc5c Gene Promoter in Colorectal Cancers

目的分析大肠癌(colorectal cancer,CRC)组织中unc5c基因启动子区域甲基化的改变状况及其临床意义。方法运用甲基化特异性PCR技术,检测73例大肠癌患者手术切除的癌组织和相应的癌旁组织及28例正常组织、36例腺瘤患者手术切除的腺瘤组织中unc5c基因启动子区域甲基化的改变情况,并分析与临床病理特征之间的关系。结果 73例癌组织样本中甲基化检出率为75%(55/73),相应的癌旁组织为7%(5/73),腺瘤组织为63%(23/36),而正常组织中未检出unc5c基因甲基化。unc5c基因甲基化检出率与年龄、分化程度及TNM分期有关。结论检测unc5c基因启动子区域异常甲基化是大肠癌早期辅助诊断的分子标志物之一。

Objective Aberrant methylation of tumor suppressor genes plays an important role in the development of colorectal cancers（CRC）. The objective of this study was to identify the epigenetic changes in CRC. Methods Methylation-specific polymerase chain reaction （MSP） analysis was used on 28 cases of normal colorectal tissues, 36 cases of adenomatous polyp tissues, 73 matched CRC tumors and adjacent normal tissues. Results The unc5c promoter was hypermethylated in 75%（55/73）colorectal cancer tissues, 7%（5/73）adjacent normal tissues and 63%（23/36） adenomatous polyp tissues, but not in normal tissues. The frequency of unc5c promoter methylation in CRC and adenomatous polyp tissues was significantly higher than that in adjacent normal tissues （P0.05）. The methylation status of unc5c gene was associated with age, histology and tumor stage（TNM）.Conclusion Our data suggested that unc5c gene promoter was frequently hypermethylated. Aberrant methylation of unc5c gene promoter may play a role in the development of CRC. Detection of the aberrant methylation of unc5c gene in tissues DNA from CRC patients might offer an effective means for the earlier auxiliary diagnosis of the malignancy.