高强度聚焦超声治疗兔乳腺移植瘤的病理变化

Pathological changes of transplanted rabbit breast tumor treated with high intensity focused ultrasound

目的:观察兔乳腺移植瘤经高强度聚焦超声(High intensity focused ultrasound,HIFU)治疗后的病理变化。方法:42只实验兔随机分成HIFU组(n=35)、对照组(n=7),HIFU组在B超监控下接受HIFU治疗,辐照后采集标本行病理检查。结果:HIFU辐照后靶区呈灰白色;即刻HE染色显示肿瘤细胞形态、排列与对照组无明显变化;电镜显示细胞膜、核膜断裂,细胞器破坏,胞质内出现无膜结构大小不等的空泡;琥珀酸脱氢酶(Succinate dehydrogenase,SDH)、增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)阴性表达,提示肿瘤细胞已死亡;随着时间的延长坏死肿瘤组织逐渐液化吸收,纤维化。结论:HIFU能有效地灭活肿瘤细胞,为HIFU开展治疗乳腺癌的临床应用提供参考价值。

Objective：To observe the histopathological changes of transplanted rabbit breast tumor treated with high intensity focused ultrasound （HIFU）.Methods：Forty-two experimental rabbits were divided into HIFU group （n=35）and control group（n=7）,HIFU ablation was completely performed in the HIFU group with ultrasound imaging guidance,and the pathological changes were examined immediately and on 1st,3rd,7th,14th,21st,28th day after HIFU treatment.Results：The target area of tumor appeared gray immediately after HIFU treatment;the morphology and arrangement of the cellular nucleus showed no obvious alteration in HE stained sections after HIFU ablation;however,electron microscopy revealed that the cellular membrane and nuclear membrane was rupturing,cell organelle destructed and appearance of vacuolus without membrane in cytoplasm;Enzyme histochemical staining showed that the succinate dehydrogenase （SDH）in target area of tumor disappeared,also the proliferating cell nuclear antigen （PCNA）was negative examined by using immunohistochemical staining,suggesting the death of tumor cells;The coagulative tissue got liquefied and absorbed with the extension of time,then it was substituted by fibrous tissue finally. Conclusion：HIFU can effectively destroy the transplanted rabbit breast tumor,it provided referred value for clinical application of HIFU in the treatment of breast cancer.