高糖对肾小管上皮细胞Toll样受体4表达及其信号通路的影响

Effect of high glucose on the expression of Toll-like receptor 4 and the signal pathway in renal tubular epithelial cells

目的:通过研究高糖对肾小管上皮细胞Toll样受体4(Toll-like receptor4,TLR4)表达及其信号通路的影响,初步探讨TLR4与高糖促进肾脏细胞分泌炎症因子的关系。方法:小鼠近曲肾小管上皮细胞(MCT细胞)在高糖环境下培养后,用Western Blot分析TLR4蛋白的表达,Western Blot和免疫沉淀法分析TLR4与髓样细胞分化因子88(Myeloid differentiation factor 88,MyD88)的相互作用,以了解TLR4信号通路是否被激活。结果:高糖可使MCT细胞TLR4蛋白的表达上调,高糖环境下MyD88依赖的TLR4信号通路被激活。结论:TLR4与高糖促进肾脏细胞分泌炎症因子有关,TLR4参与糖尿病肾病的发病。

Objective：To explore the role of Toll-likereceptor 4 in the production of inflammatory factors in renal innate cells stimulated with high glucose by studying the effect of high glucose on the expression of TLR4 and its signal pathway. Methods：Western Blot analysis was taken to measure the expression of TLR4,and the combination of Western Blot and immunoprecipitation was used to analyze the correlation of TLR4 with MyD88. Results：High glucose upregulated the expression of TLR4 protein and activated MyD88-dependent pathyway in MCT cells. Conclusion：TLR4 is involved in the pathogenesis of diabetic nephropathy by playing a role in the production of inflammatory factors in renal innate cells stimulated with high glucose.