黄芪对兔耳增生性瘢痕作用的实验研究

The effects of radix astragali on hypertrophic scars in rabbit ears

目的:观察黄芪(Radix astragali)对日本大耳白兔增生性瘢痕的作用。方法:日本大耳白兔20只,建立兔耳增生性瘢痕动物模型。待创面上皮化(伤后21d)将其随机分为5组,每组4只,实验组A、B、C分别局部注射不同浓度的黄芪注射液,对照组D局部注射生理盐水,对照组E为空白对照。分别在伤后32、43d测量瘢痕厚度、硬度,并常规做HE染色,RT-PCR观察瘢痕组织中转化生长因子β1(Transforming growth factor-β1,TGF-β1) mRNA水平的变化,免疫组化观察TGF-β1蛋白的表达。结果:治疗组中瘢痕厚度、硬度与药物浓度呈负相关(P<0.05),治疗组瘢痕组织中TGF-β1 mRNA和其蛋白水平均明显减少,呈剂量效应关系;与生理盐水和空白对照组比较,差异有统计学意义,实验组在伤后32、43d时两组间差异有统计学意义(P<0.05),对照组在伤后32、43d时两组间差异无统计学意义(P>0.05)。结论:黄芪注射液通过减少TGF-β1 mRNA水平合成,减少TGF-β1蛋白的表达,从而抑制兔耳增生性瘢痕组织增生,显示黄芪注射液可能是治疗增生性瘢痕较好的药物。

Objective：To observe the effects of radix astragali on hypertrophic scars in rabbit ears. Methods：Twenty healthy adult Japanese big ear rabbits were given hypertrophic scars over the ventral surface of the ears and on the 21st day after wound,animals were randomly divided into the treated groups （groups A,B,and C were treated with radix astragali in different concentrations）and the control groups（groups D and E were treated with saline and blank control,respectively）. On the 32nd and 43rd day after wound,the thickness and hardness of scar were measured.The TGF-β1mRNA were detected in hypertrophic scar samples with RT- PCR.The change of scar tissues was observed with HE staining and immunohistochemical staining with TGF-β1. Results：The thickness and hardness of scar were inversely correlated with the concentration of radix astragali in the radix astragali-treated group（P〈0.05）.Image analysis demonstrated that the expression of TGF-β1mRNA and its protein in all radix astragali-treated groups were significantly reduced as compared withthose in saline group and blank control group;There was significant difference in treated groups（P〈0.05）,but no significant difference in control groups （P〉0.05）between the 32nd day and43rd day groups.Conclusion：Radix astragali could inhibit proliferation of hypertrophic scars in the rabbit ears by decreasing the expression of TGF-β1mRNA and its protein. Thereby radix astragali may have beneficial effects in the treatment of hypertrophic scar.