成骨生长肽对钛表面人牙周膜成纤维细胞增殖分化影响的研究

Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.

目的:研究成骨生长肽(Osteogenic growth peptide,OGP)对人牙周膜成纤维细胞(Periodontal ligament cells,PDLCs)在钛金属表面增殖分化的影响。方法:将纯钛试件放在12孔培养板内,取生长良好的第五代人牙周膜成纤维细胞接种在试件表面,加入不同浓度的OGP(10-11～10-7mol/L),分别在接种后1、3、5、7d应用MTT法检测细胞增殖;接种后3、7、10d应用酶联免疫法检测细胞内碱性磷酸酶(Alkaline phosphatase,ALP)活性的表达。结果:OGP在该浓度范围内时,对钛表面的人牙周膜成纤维细胞的增殖率有明显的促进作用(P<0.05),并且能促进细胞内ALP表达活性(P<0.05),最佳作用浓度为10-9mol/L。结论:OGP可促进钛片表面人牙周膜成纤维细胞的增殖活性,同时可以增强细胞碱性磷酸酶表达的活性,提示OGP在口腔种植领域中具有良好的应用前景。

Objective：To elucidate the effect of osteogenic growth peptide （OGP） on proliferation and differentiation of human periodontal ligament cells on titanium disc in vitro. Methods：Human periodontal ligament cells were cultured located on titanium disc individually in medium contained various concentrations of OGP;and cell proliferation quantity were examined by MTT and alkaline phosphatase activity were examined by enzyme-linked immunosorbentassay. Results：In vitro,the addition of OGP in cultures resulted in an increase of cell proliferation and ALP activity（P〈0.05）. All OGP concentrations used （10^-11-10^-7mol/L）were effective but the maximum response was obtained with 10-9mol/L OGP. Conclusion：Our results demonstrate that OGP stimulates proliferation and ALP expression of human periodontal ligament cells located on titanium disc in vitro and suggest that OGP is a promising growth factor in the region of oral implant.