新疆维吾尔族胆固醇结石患者肝组织SCP_2蛋白的表达

Expression of sterol carrier protein 2 gene in Urgur patients with cholesterol gallstone in Xinjiang

目的研究新疆维吾尔族(维族)与汉族胆囊胆固醇结石患者及维族非结石患者肝组织固醇携带蛋白2(SCP2)表达和血脂变化情况。方法应用Western blot技术,研究新疆维族胆囊胆固醇结石患者、汉族胆固醇结石患者和维族非结石患者各20例肝组织SCP2的蛋白表达水平差异;并采用生物化学技术检测其血脂改变。结果维族与汉族胆囊胆固醇结石患者肝组织SCP2蛋白表达水平差异无统计学意义,维族胆囊胆固醇结石患者肝组织SCP2蛋白表达水平较维族非结石患者升高,差异有统计学意义;各组患者血脂差异无统计学意义。结论新疆维族胆囊胆固醇结石患者肝组织SCP2蛋白水平升高,SCP2表达上调可能是通过影响血脂代谢参与胆囊胆固醇结石的形成。

Objective To investigate the expression level of liver SCP-2 protein and serum lipid level in Uygur and Han patients with cholesterol gallstone and Uygur patients without gallstones in Xinjiang and changes of serum lipid levels.Methods The expression level of liver SCP-2 protein was studied in 20 Uygur patients and 20 Han patients with cholesterol gallstoneby Western blot method,20 Uygur patients without gallstones as control group.The serum lipid levels were also determined by biochemical techniques.Results There were no significant differences between 20 Uygur patients with cholesterol gallstone and 20 Han patients with cholesterol gallstone,however.The expression of SCP-2 protein was increased significantly in Uygur patients with cholesterol gallstone in comparision with Uygur patients without gallstone.There were no significant differences in the serum lipid levels among different groups.Conclusion SCP-2 gene was overexpressed in cholesterol gallstone Uygur patients with cholesterol gallstone in Xinjiang,indicating that SCP-2 may take part in developing cholesterol gallstone through affecting metabolism of lipids（because there are not different in serum lipid level,so you can not draw such conclusion）.