MHC-I蛋白表达与维吾尔族妇女宫颈病变进程的关系

The association of expression of MHC-I protein with the cervical lesion pathogenesis in Uighur women

目的观察人主要组织相容性抗原I(major histocompatibility complex,MHC-I)表达缺陷与维吾尔族妇女宫颈癌前病变进程的关系。方法收集维吾尔族妇女宫颈炎、宫颈内上皮瘤样病变(cervical intraepithelialneoplasia,CIN)和宫颈鳞癌患者的石蜡包埋组织标本共150例,采用免疫组织化学SP法检测MHC-I蛋白表达水平。结果 MHC-I抗原在慢性宫颈炎、CIN、宫颈鳞癌组织中阳性表达逐渐减少,其差异有统计学意义(P<0.001),并随着宫颈病变的进展呈下降趋势(r=-0.656,P<0.001);在宫颈上皮内瘤样病变中,MHC-I在宫颈上皮细胞表面表达逐渐减少,差异有统计学意义(P<0.01);MHC-I的表达与宫颈癌临床分期、病理分化程度、淋巴结转移密切相关。结论 MHC-I抗原的表达和异型细胞的恶性程度呈负相关趋势,表明MHC-I表达在宫颈上皮内瘤变和宫颈癌的发生、发展中可能起着重要的保护作用。

Objective To investigate the association between the loss of expression in major histocompatibility complex（MHC-I） and the cervical precancerous lesion pathogenesis in Uighur women.Methods It was collected 150 samples of formalin fixed and paraffin-embedded cervical tissue specimens from Uighur women with cervicitis,cervical intraepithelial neoplasia（CIN） and cervical squamous cell carcinoma,and detected the protein expression level of MHC-I by SP assay of immunohistochemistry.Results The expression of MHC-I is gradually reduced in Uighur women with cervicitis,CIN,SCC.The difference among hem had statistic difference（P0.001）.The expression of MHC-I had a negative correlation with the cervical diseases（r=-0.656,P0.001）.The expression of MHC-I gradually reduced in Uighur women in CIN.The difference among hem had statistic difference（P0.01）.The expression of MHC-I was closely associated with the clinical classification,differentiation state,and lymph node metastasis.Conclusion The expression of MHC-I has a negative correlation with the degree of heterocellular malignancy.It indicates MHC-I may play important roles in the development and progression of CIN and cervical cancer.