摘要
采用抑菌圈法研究了壳聚糖对大肠杆菌(E.coli)和金黄色葡萄球菌(St.aureus)的抑菌活性。利用壳聚糖的席夫碱反应,对壳聚糖的胺基进行保护后,研究了壳聚糖中胺基对其抑菌性能的影响。同时,运用紫外吸收光谱和电化学的方法,研究了壳聚糖与DNA的相互作用,提出了壳聚糖对E.coli和St.aureus的抑菌机理。研究结果表明,壳聚糖对E.coli和St.aureus具有很好的抑制作用,且抑菌活性与其胺基有关;壳聚糖能与细胞内带负电的核酸结合,使细胞正常DNA复制生理功能受到影响,抑制细菌的繁殖,从而达到抑菌的目的。
The antibacterial activity of chitosan against Escherichia coli and Staphylococcus aureus were investigated by measuring inhibition zone. Aimed to explain the effect of amino group on antibacterial activity of ehitosan, four different kinds of chitosan Schiff＇ s bases were synthesized. UV absorption spectroscopy and electrochemical analysis were used to illuminate the interaction of chitosan with DNA. The results showed that the antibacterial ability of chitosan was related to the amino group. Chitosan can bind with negative nucleic acids in eell, affeet the normal replication function of DNA and ultimately inhibit the growth of bacteria.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第3期373-377,470,共6页
Natural Product Research and Development
基金
甘肃天水师范学院物理无机化学重点学科基金
关键词
壳聚糖
抑菌活性
希夫碱
胺基
DNA
chitosan
antibacterial activity
Schiff＇ s base
amino group
DNA
