绿豆和乳清蛋白水解物对双歧杆菌促生长条件的研究

Studies on the Characters of Growth Condition for the Bifidobacterium Using the Hydrolysate of the Mung bean Albumen

对婴儿双歧(Bifidobacterium infantis)和两歧双歧杆菌(Bifidobacterium bifidum)的生长发育特性进行研究,在掌握双歧杆菌基本特性的基础上,以乳糖、乳清水解物和绿豆水解物为促生长因子,研究其对双歧杆菌的增殖影响。经过单因素和正交试验发现,乳清蛋白水解物和绿豆蛋白水解物对两种双歧杆菌都有显著的促生长作用。最佳培养基优化方案为:对婴儿双歧杆菌,在基础PTYG培养基基础上,添加1.5g/100mL的乳糖,40%的乳清蛋白水解物和40%的绿豆蛋白水解物;对两歧双歧杆菌,在基础PTYG培养基基础上添加0.5g/100mL的乳糖,40%的乳清蛋白水解物和40%的绿豆蛋白水解物。

This paper researched on the characters of growth for Bifidobacterium infantis and Bifidobacterium bifidum,and based on the basic characters of Bifidobacterium,found a kind of multiplicating culture medium,which is suited for different strains of Bifidobacterium,with comparing the effects of lactose,hydrolysate of the whey albumen and that of the mung bean albumen as growth factors on Bifidobacterium.The hydrolysate of the whey albumen and the mung bean albumen can promote the growth of both remarkably.The best scheme of the culture medium is：1.5 % of lactose,40 % of hydrolysate of the whey albumen and 40 % of hydrolysate of the mung bean albumen for B.infantis,and 0.5 % of lactose,40 % of hydrolysate of the whey albumen and 40 % of hydrolysate of the mung bean albumen for B.bifidum.