用于研究快速扫视对瞳孔光反射调制作用的新方法

A New Method to Explore the Modulation from the Saccadic System on the Pupillary Light Reflex System

瞳孔与光反射系统和快速扫视系统在解剖学和功能上都有着紧密联系,但是快速扫视系统对瞳孔的光反射系统是否有调制作用尚无报道。研究这两个系统间的调制作用,必须了解光刺激不均匀和近反应对瞳孔直径变化是否有影响。该研究以人为被试,设计了一种全新的实验方法,研究光刺激不均匀和近反应对瞳孔直径变化的影响。实验方法:将被试的一只眼用密闭的眼罩罩住给予脉冲光刺激,刺激由位于眼罩内全视野范围水平排列的一排发光二极管(lightemitting diodes,LEDs)给出,被试的另一只眼用来记录眼动和瞳孔直径的变化,研究水平方向的快速扫视对瞳孔对光反射时瞳孔直径变化的影响。实验结果:比较被试注视视野内不同位置的瞳孔对光反射相对收缩率无显著差异(P=0.148,非配对样本t检验)。结论:该方法消除了光刺激不均匀和近反应对瞳孔直径变化的影响,可用于研究快速扫视系统对瞳孔光反射系统间的调制作用。

The saccadic system has anatomical and functional connections with the pupillary light reflex （PLR） system. But it is not known whether the saccadic system modulates the PLR system. To investigate this issue, it is necessary to understand whether the uneven light stimulus to retina and the near responses influence the change of pupil diameter. We designed a new behavioral method to investigate the issue on human subjects. Methods： one eye of the subject was stimulated by pulse light stimulus from a horizontal linear array of light emitting diodes （LEDs） presented across visual field in an ocular mask. The changes of the eye position and pupil diameter of another eye were recorded by an infrared eye tracking system. Results： The relative constriction ratios of PLRs on the condition that the fixation points of subjects were in the nasal visual field were not significantly different from that the fixation points of subjects were in the temporal visual field（P=0.148, non-paired t test）.Conclusion： The influences from uneven light stimulus to retina and the near responses were eliminated by this method. The method can be used to study the modulation from the saccadic system on the pupillary light reflex system.