冬春不同栽培环境下‘宁海白’枇杷幼果酚类物质含量与代谢的变化

Phenolic compounds and its metabolism during winter and spring in young loquat fruit of 'Ninghaibai'

以露地、套袋和大棚栽培的‘宁海白’枇杷为试材,测定了低温期间和低温解除后的幼果内总酚含量及与酚类物质代谢相关的苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化物酶(POD)活性变化。结果表明,在低温期间,露地栽培的枇杷幼果总酚含量显著高于套袋与大棚栽培的幼果;低温结束后露地栽培枇杷幼果的总酚含量虽然高于大棚与套袋果实,但比低温期间明显下降。酚类物质代谢相关酶分析显示,低温期间PAL活性显著高于低温解除后的果实,露地幼果活性高于套袋与大棚;PPO活性低温期间以露地栽培的果实为最低、套袋处理最高,低温结束后以大棚为最低、套袋为最高;POD活性低温期内以露地为最高、大棚最低,低温解除后活性显著下降,但大棚栽培要低于露地与套袋。以上结果表明,酚类物质积累和代谢与枇杷抵抗低温胁迫的响应有关。

The total phenolic content and activities of phenylalanine ammonia-lysae（PAL）,polyphenol oxidase（PPO）,peroxidase（POD） in fruits of ＇Ninghaibai＇ loquat（Eriobotrya japonica Lindl） cultivated in open air,bagging and greenhouse were measured in winter and spring.The results showed that total phenolic content in fruit cultivated in open air condition was significantly higher than those in bagging and greenhouse conditions in winter and decreased with the coming of spring.However,the total phenolic content in fruit cultivated in bagging and greenhouse conditions almost unchanged between winter and spring.The PAL activities of fruits in all treatments in winter were much higher than those in spring,and the activity in open air was higher than those in bagging and greenhouse.Activity of PPO in fruit of open air was the lowest in winter;the highest was in bagging both in winter and spring.The activity of POD in open air was higher than those in bagging and greenhouse in winter.In spring,the activities of POD decreased.These results suggested that the accumulation and metabolism of phenolic compounds might be related to the resistance of freeze in loquat fruit in winter.