抗独特型抗体在小分子农药、真菌毒素免疫检测中的应用

Review on application of anti-idiotype antibodies in immunoassay for pesticides and mycotoxins

小分子农药、真菌毒素免疫分析技术的发展,存在难以实现多残留检测、标准品昂贵且不易获得和检测灵敏度受限制等制约因素,而抗独特型抗体技术可以为这些问题的解决提供新的思路。文章阐述了抗独特型抗体的基本原理,该技术在小分子食品污染物免疫分析技术中的应用及优势,国内外在此领域的研究进展,以及该技术存在的制约因素与发展前景。

There are some bottlenecks in the development of immunochemical analysis for pesticides and mycotoxins,such as multi-residue analysis,the cost and availability of standard,and limit of sensitivity.However,the anti-idiotype antibodies（AId） have provided some new approaches to solve these problems.In this review,the principles of anti-idiotype antibodies were introduced and the special application advantages of AId in detection of small molecular contaminate in food were discussed.Current applications,restrictions and prospect of AId in these fields were also exemplified.