白血病细胞系K562上肾上腺素受体检测及其类型分析

Detection of adrenergic receptors on K562 cells and analysis of type

目的:观察白血病细胞系K562上是否存在肾上腺素受体及对其类型进行分析。方法:用荧光标记的肾上腺素处理K562细胞后,采用紫外分光光度计和荧光分光光度计检测。结果:荧光标记的肾上腺素处理后的K562细胞A值和F值显著高于对照组。受体阻断结果显示:α、β受体均起作用。结论:K562细胞上同时存在α和β肾上腺素两种类型的受体。

Objective：To investigate whether there are adrenergic receptors on K562 cells,and analysis the subtype of adrenergic receptors on K562 cells.Methods：K562 cells were treated with fluorescent labelled adrenalin.After treatment,K562 cells were detected with fluorescence spectrophotometer and UV spectrophotometer.Results：A value and Fvalue of K562 cells after the treatment with fluorescent labelled adrenalin were obviously higher than that of the control group,research of receptors blocked suggested that both α andβadrenergic receptors all had the action.Conclusion：There are bothα andβadrenergic receptors on K562 cells.