SD大鼠骨骼肌卫星细胞系的建立及诱导其向成肌、成脂和成骨分化

Establishment of SD rat skeletal muscle satellite cell line and its differentiation into myogenic, adipogenic and osteogenic cells~

目的：建立SD大鼠骨骼肌卫星细胞的细胞系，为组织工程学提供种子细胞和研究资料。方法：采用两步酶消化法结合差速贴壁技术，获得较高纯度的骨骼肌卫星细胞。MTT法观察骨骼肌卫星细胞的体外增殖特性并绘制生长曲线图，流式细胞仪检测细胞周期。观察不同诱导条件下骨骼肌卫星细胞的成肌、成脂、成骨的分化特性。结果：骨骼肌卫星细胞可传50代以上，第7代时，倍增时间约为60h，80．7％的细胞处于G1期，在特定诱导作用下骨骼肌卫星细胞可以向成肌、成脂、成骨方向分化，显示了骨骼肌卫星细胞的多向分化潜能，成功建立了骨骼肌卫星细胞的细胞系。结论：建立了SD大鼠骨骼肌卫星细胞系，为组织工程学提供种子细胞。

Objective： To establish SD rat skeletal muscle satellite cells （SMSCs） cell line that gives seeded cells for tissue engi neering. Methods： Twice digestion method and differentiation adhesion time method were used for the harvest of SMSCs. MTT was used for investigating growth character of SMSKTs and drafting the growth curve. Cell cycle was detected by flow cy- tometry and the character of SMSKTs differentiation into myogenic, adipogenic and osteogenic cells in differentiation culture was investigated. Results： Passages were conducted over 50 times. In the 7th passage, doubling time was approximately 60 hours, and 80.7% of cells were in G1 phase. SMSCs of SD rat were induced to differentiate into myogenic, adipogenic and osteogenic cellls to illustrate its multipotentiality. Conclusion： The cell line of SD rat SMSCs was established. SMSCs can be used as seed cells for tissue engineering.