白细胞介素-6在反复自然流产患者绒毛和蜕膜组织中的表达

Expression of interleukin-6 in villi and decidua of patients with recurrent spontaneous abortion

目的：研究白细胞介素-6（IL-6）在反复自然流产患者绒毛与蜕膜组织中的表达情况，为防治反复自然流产提供理论依据。方法：应用免疫组织化学显色法和图像分析技术，对IL-6蛋自在反复自然流产患者（病例组）和正常人工流产者（对照组）绒毛和蜕膜组织中的表达进行定位和半定量分析；H—E染色法观察反复自然流产患者绒毛和蜕膜组织的形态学变化。结果：免疫组织化学显色显示，在绒毛组织中，IL-6蛋白定位于滋养层细胞的细胞质内，病例组的IL-6蛋白表达明显低于对照组；在蜕膜组织中，IL-6定位于蜕膜细胞和腺上皮细胞的细胞质内，且病例组蜕膜细胞的表达明显高于对照组，而2组在腺上皮细胞的表达无差异；H—E染色结果显示，病例组绒毛组织的滋养层变薄，细胞变性、坏死，细胞嗜酸性增强，绒毛中轴纤维化程度增强，蜕膜细胞失去细胞间连接。部分蜕膜细胞解体、核消失，细胞嗜酸性增强。结论：绒毛和蜕膜组织中IL-6蛋白表达的变化与反复自然流产有关．IL-6可能参与反复自然流产的病理过程。

Objective： To study the expression of Interteukim-6 （IL-6） in villi and decidua of patients with recurrent spontaneous abortion （RSA） and to reveal the pathogenesis and offer the definite theoretical basis for the prevention and cure of patients who suffer from RSA. Methods： With immunohistochemistry method and image analysis technology, the local and quantitative expressions of IL-6 were detected and analyzed between the patients of RSA （case group） and the patients of normal induced abortion （control group）. The morphological change of villi and decidua in RSA was observed under a light microscope by hematoxylin-eosin staining. Results： Immunohistochemistry staining showed IL-6 protein located in cytoplasm of trophoblast cells in villi, and the expression of IL-6 protein in case group was distinctively lower than that of in the control group; In decidua, IL- 6 located in cytoplasm of decidua cells and glandular epithelial cells, the expression of decidua cells in the case group was significantly higher than that of in the control group. There was no difference between two groups in glandular epithelial cells. Trophoblast became thinner, cells degenerated and necrosed, cells turned more acidophily, and the fibration of villous axis increased in the case group. There was connection between decidua cells. Some portion of decidua cells dissolved and their nucleus disappeared, and eosinophilic strengthened. Conclusion： In the case group, IL-6 protein in expression of villous and decidua maybe correlate with RSA, which may take part in the pathological process of RSA.