氢饱和生理盐水对慢性氧中毒肺、脑组织形态学的影响

Effects of saturated hydrogen saline on histomorphology of the lung and brain after chronic oxygen toxicity

目的：研究氢饱和生理盐水对慢性氧中毒的预防保护效应。方法：新生7dSD大鼠于常压95％的氧中暴露24h，制备慢性氧中毒模型。暴露过程中每6h腹腔注射生理盐水或不同剂量含氢生理盐水。采用肺H-E、脑H—E和Nissl染色分别观察含氢生理盐水对慢性氧中毒时肺和脑组织的保护效应。结果：H—E染色显示，吸氧后生理盐水组小鼠肺毛细血管显著扩张、出血严重，支气管上皮变形明显，且肺间质和肺泡腔内可见大量红细胞，H—E及Nissl染色显示，脑神经元明显水肿和变性；氢饱和生理盐水组小鼠肺血管出血、支气管上皮变形以及炎症反应严重程度有所缓解，脑部神经元水肿和变性严重程度有所减轻，神经元的数量增加。结论：氢饱和生理盐水对慢性氧中毒肺及脑损伤具有一定预防作用。

Objective： To investigate the effects of saturated hydrogen saline on normobaric oxygen induced chronic oxygen toxicity. Methods： Seven-day-old Sprague-Dawley （SD） rat pups were exposed to normobaric oxygen （95%） for 24 h to develop chronic oxygen toxicity. The animals with chronic oxygen toxicity were intraperitoneally administrated with normal saline or hydrogen saturated saline of different doses every 6 h during exposure （a total of 4 times）. Pathological changes in the lung and the brain were evaluated by H-E staining and Nissl staining plus H-E staining, respectively. Results： Our results revealed that pulmonary damages in the hydrogen saline treated pups were significantly improved when compared with the saline treated pups. Saturated hydrogen saline could markedly alleviate pathological lesions in the lung tissue, ease inflammatory cell infiltration and decrease total cells; alleviate neuron edema and degeneration, and increase neuron number. Conclusion： Saturated hydrogen saline could prevent pulmonary and neuronal lesions induced by normobaric oxygen.