大鼠多重脑震荡基底前脑胆碱能与5-羟色胺能神经的变化

Interaction between cholinergic neurons forebrain following multiple and serotoninergic nerve fiber in basal cerebral concussion in rats

目的：探讨体育运动中较为多发的多重脑震荡（MCC）后的脑损伤机制。方法：用金属单摆闭合性脑损伤打击装置复制MCC大鼠模型，伤后分1-24d多个损伤组．用免疫组织化学技术研究基底前脑5-羟色胺（5-HT）能与胆碱能神经元的变化。结果：脑震荡后随胆碱乙酰转移酶（CHAT）免疫阳性表达的降低，色氨酸羟化酶（TPH）免疫阳性表达升高，随损伤后时间的延长，ChAT表达逐渐升高．TPH表达也随之下凋趋正常。结论：多重脑震荡后，与认知密切相关的不同脑区在5HT神经元表达增强的同时．胆碱能神经元呈下调改变．这些变化可能与脑损伤后的认知行为障碍有关。

Objective： To investigate the effect of brain injury after multiple cerebral concussion in sports. Methods： We studied the histopathologic changes of cerebral neurons in multiple cerebral concussions rats which were duplicated using a metallic pendulum-striker concussive device. Forty-two male SD rats were randomly diveded into seven groups： control group, and some post-injury for 1-24 d groups （n= 6 for each group）. The expression of serotoninergic and eholinergic nerve in the different brain area was also detected and quantitated by image analysis system after iramunohistological double staining. Results： The expression of ChAT was of decreasing tendency in different brain areas post-MCC. At the same time, expression of TPH was ascending after injury. Conclusion： 5-HT can be related to the recognize function by acetylcholine.