人工感染边缘无浆体对犊牛血清生化及血液流变学的影响

Effects of artificial Anaplasma marginale infection on biochemical and hemorheological parameters in calf

为通过人工感染的方法建立牛边缘无浆体病动物模型,研究边缘无浆体(A.marginale)感染对犊牛血液主要生化指标和血液流变学指标的影响,本实验采用地塞米松注射液长期抑制一月龄犊牛的免疫力,经颈静脉接种含有A.marginale的血液,定期采血,测定其血液主要生化指标和血液流变学指标。结果表明,感染A.marginale后,血液主要生化指标与对照组相比,谷丙转氨酶(GPT)含量极显著升高(p<0.01),谷草转氨酶(GOT)含量显著升高(p<0.05),血糖(GLU)含量显著降低(p<0.05),总胆固醇(T-CHO)含量极显著降低(p<0.01),而碱性磷酸酶(ALP)、血铁(Fe)、尿素(BUN)、乳酸脱氢酶(LDH)、α-羟丁酸脱氢酶(α-HBD)和肌苷(Cr)含量均无显著性变化;血液流变学主要指标与对照组相比,全血还原高切比粘度和红细胞刚性指数(IR)极显著降低(p<0.01),全血中切比粘度、全血低切比粘度和全血还原低切比粘度显著降低(p<0.05),全血高切比粘度、血浆比黏度(ηp)、红细胞压积(PCV)和红细胞聚集指数(AI)均无显著性变化。

An artificial model of Anaplasma marginale infection in calf was established under immune suppression conditions. One-year old calf was treated with dexamethasone for a week at least and then injected intravenously with the A.marginale infected blood. Samples were collected and the blood biochemical and hemorheological parameters analyzed. Results showed that the glutamate pyruvate transaminase level and glutamic-oxal acetic transaminase level of the sera in the A.marginale infected calf was significantly higher than those of the control animals （p〈0.01）, while the total cholesterol level and glucose level was markedly lower （p〈0.05）. However, alkaline phosphatase, errum, blood urea nitrogen, lactate dehydrogenase L, a -hydroxybutyrate dehydrogenase and chromium levels were not significantly different （p〉0.05）. The hemorheological parameters such as η r （high） and IR, ηb30 s-1, ηb1 s-1 and ηr （low） levels were also lower than control （p〉0.05）, but ηb200 s-1, ηp, PCV and AI levels were not significantly different （p〉0.05）.