狼疮性肾炎患儿外周血CD4^+CD25^+调节性T细胞的变化

Changes of CD4^+CD25^+Tregs in peripheral blood of patients with lupus nephritis

目的探讨狼疮性肾炎(LN)患儿外周血CD4+CD25+调节性T细胞(Tregs)的变化及意义。方法采用流式细胞术检测30例LN患儿(LN组)和30例健康儿童(对照组)外周血CD4+CD25+调节性T细胞百分比,并分析其与24-h尿微量白蛋白(UMA)及狼疮活动指数(SLE-DAI)的关系。结果 LN患儿外周血中CD4+CD25+Tregs明显低于对照组(P<0.01);CD4+CD25+Tregs与24-hUMA呈显著负相关,与SLE-DAI呈等级负相关。结论 LN患儿外周血CD4+CD25+Tregs百分比异常降低可能参与LN的起病与发展。CD4+CD25+Tregs百分比与24-hUMA呈显著负相关,与SLE-DAI呈等级负相关,提示可以作为监测LN患儿病情及预后的指标。

Objective To investigate the changes of CD4＋CD25＋Tregs in peripheral blood mononuclear cell（PBMC）in patients with lupus nephritis（LN）.Methods Flow cytometry technique was used to detect CD4＋CD25＋Tregs in 30 LN children（group A）and 30 healthy children（group B）.Relationships of the changes of CD4＋CD25＋Tregs with 24-h urine microalbumin（UMA）and SLE-DAI were analyzed.Results Relative mean fluorescence intensity（RMFI）of CD4＋CD25＋Tregs was lower in group A than that in group B（P0.01）.The level of CD4＋CD25＋Tregs was negatively correlated to 24-h UMA and SLE-DAI.Conclusion The abnormal low level of CD4＋CD25＋Tregs in PBMC of LN children may play a pathological role in the development and progression of LN.The level of CD4＋CD25＋Tregs is negatively correlated to 24-h UMA and SLE-DAI,which indicats that CD4＋CD25＋Tregs may be taken as an immunological marker for evaluating the severity and prognosis in the patients with LN.