三羟基异黄酮对ACC2及ACCM细胞株侵袭能力的影响

Effect of Genistein on the Invasiveness of ACC2 and ACCM Cell Lines in vitro

目的:观察4,5,7-三羟基异黄酮(Genistein)对腺样囊性癌细胞株(ACC2)及腺样囊性癌肺转移株(ACCM)的侵袭能力,和对两细胞株金属基质蛋白酶-2(MMP-2)蛋白表达的影响。方法:采用细胞划痕实验及Transwell小室侵袭实验,检测Genistein对ACC2和ACCM细胞侵袭能力的影响;采用明胶酶谱法及流式细胞术分别检测Genistein对两种细胞株MMP-2蛋白胞外分泌及胞内表达的影响。结果:Genistein能显著抑制ACC2及ACCM两种细胞株的侵袭能力(P<0.01),但两细胞株之间不存在差异(P>0.05)。同时,Genistein可使两细胞株细胞外分泌MMP-2蛋白的水平下调,及胞内MMP-2蛋白的表达降低(P<0.05)。结论:Genistein可对ACC2及ACCM的侵袭产生明显的抑制作用,该作用可能与诱导胞内外侵袭相关蛋白MMP-2的表达降低有关。

Objective： To study the effect of Genistein on the invasion of human adenoid cystic carcinoma ACC2 and ACCM cell lines in vitro. Methods： Effects of migration and invasion were assayed by the methods of transwell migration assay and scrape wound healing assay. The levels of MMP-2 in the ecto-medium were detected by gelatin zymography and gel imaging system. The MMP-2 level of the tumor cells was detected by flow cytometry. Results： The inhibitory effect on invasion occurred in the Genistein group was more potent compared with the control group （P〈0.01）. Genistein could decrease the expression of MMP-2 by 29.81% and decrease the expression of activated MMP-2 by 17.63% in the ecto- medium of ACC2. It could also decrease the expression of MMP^2 by 41.25% and the expression of activated MMP-2 by 29.89% in ACCM. Genisitein significantly inhibited the expression of MMP-2 in tumor cells by the flow cytometry. Conclusion： Genistein significantly depresses the invasion of ACC2 and ACCM in vitro, and decreases the intracellular and extracellular MMP-2 expression.