摘要
研究以甘薯淀粉为原料,接种热带假丝酵母(Candida.tropicalis),采用液态发酵法生产单细胞蛋白,对发酵工艺展开了优化研究。结果表明,最佳发酵工艺条件为:还原糖浓度为9%,装液量为40mL/250mL,初始pH为4.5,发酵时间为54h,温度为30℃,转速为140r/min～160r/min。在此培养条件下收获的酵母细胞干重为10.35g/L,蛋白含量为51.36%,总蛋白得率为5.32g/L。本研究为甘薯利用提供了新的途径。
Sweet potato amylum was used as material to produce single cell protein by liquid fermentation with inoculant Candidca tropiccdis. The fermentation technologies were studied here. The results shouwed that： during fermentation, the perfect proportion of reducing sugar was 9%, filling volume 30ml/250ml, initial pH 4.5, through a 54h culture at 30℃ with rocking at 140-160r/min. And the produced cell dry weight of yeast was 10.35g/L, protein content 51.36% and total protein yield 5.32g/L. The research will afford a new approach for utilizing sweet potato.
出处
《食品与发酵科技》
CAS
2010年第3期68-71,共4页
Food and Fermentation Science & Technology
关键词
甘薯淀粉
液态发酵
单细胞蛋白
工艺优化
总蛋白得率
sweet potato amylum
liquid fermentation
single cell protein
technology optimization
total protein yield
