肥胖抑制素对大鼠心室肌细胞收缩及L-型钙通道的影响

Effects of obestatin on contraction of rat ventricular myocytes and L-type Ca^(2+) current

目的探讨肥胖抑制素(obestatin)对心肌细胞收缩、细胞内钙瞬变及细胞膜L-型钙通道的影响。方法采用单细胞收缩/荧光边缘探测系统及全细胞膜片钳技术观察obestatin对急性酶解分离大鼠心室肌细胞收缩及钙信号的影响。结果 10-7mol/Lobestatin增强大鼠心室肌细胞收缩幅度[(26.43±6.13)%]及细胞内钙瞬变幅度[(21.33±4.73%)],并增强ICa-L峰值。结论 Obestatin开放心肌细胞膜上L-型钙通道,促进钙内流,增强大鼠心室肌细胞ICa-L及细胞内钙瞬变,进而增强大鼠心肌细胞收缩。

Objective To study the effects of obestatin on the contraction,calcium transient and L-type Ca2＋ current （ICa-L） in rat ventricular myocytes. Methods The extent of contraction and Ca2＋ transient changes were recorded with the motion edge detection system,and L-type Ca2＋ current was recorded with the whole cell patch-clamp technique. Results Obestatin of 10-7mol/L increased the contraction amplitude of ventricular myocytes （26.43±6.13）% and Ca2＋ transient amplitude （21.33±4.73）%,and enhanced ICa-L peak. Conclusion Obestatin can strengthen contractility through increasing ICa-L and Ca2＋ transient in rat ventricular myocytes.