摘要
为探讨吸入性甲醛能否对小鼠骨髓造血细胞产生遗传毒性,以SPF级昆明雄性小鼠为材料,采用动态吸入方式连续染毒72h,取骨髓细胞,测定DNA-蛋白质交联.结果发现,随着甲醛浓度的升高(0、0.5、1.0、3.0mg·m-3),小鼠骨髓细胞DNA-蛋白质交联系数逐渐升高,0.5mg·m-3组与对照组存在显著差异(p<0.05),1.0、3.0mg·m-3组与对照组存在极显著差异(p<0.01).表明,在实验浓度范围内,甲醛对小鼠骨髓细胞具有一定的遗传毒性.
In order to study the genotoxicity of gaseous formaldehyde（FA）on mouse bone marrow cells,SPF level Kunming male mice were exposed to gaseous formaldehyde for 72h.KCl-SDS assay were applied to detect DNA-protein crosslinks（DPC）in bone marrow cells.Results showed that low concentration（0.5mg·m-3）gaseous formaldehyde could induce DPC formation significantly（compared with the control,p0.05）,and high concentrations（1.0,3.0mg·m-3）could induce DPC formation very significantly（p 0.01）.DPC was increased with the increasing of gaseous formaldehyde concentrations（0.5,1.0,3.0mg·m-3）.The results suggested that among the concentrations investigated in the present study,gaseous formaldehyde had obvious genetic toxicity on the mouse bone marrow cells.
出处
《生态毒理学报》
CAS
CSCD
2010年第2期262-267,共6页
Asian Journal of Ecotoxicology
基金
国家科技支撑计划项目(No.2006BAJ02A10)
