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鹅细小病毒结构蛋白B细胞线性抗原表位的鉴定 被引量：1

Localization of Linear B-cell Epitopes on Goose Parvovirus Structural Protein
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摘要 为了对鹅细小病毒结构蛋白B细胞线性抗原表位进行进一步定位,设计了16个覆盖结构蛋白各抗原表位区的长约30个氨基酸残基的重叠短肽,并进行了融合表达。用攻毒10周龄鹅血清对这16个融合蛋白进行蛋白质印迹分析,检测结果表明,VP蛋白线性抗原表位位于35-81、111-161、423-453、462-491、531-577、616-669和678-732氨基酸区域。 In order to determine the B-cell epitopes on the structural protein（VP） of goose parvovirus,the epitopes were dissected into 16 fragments, and expressed in Escherichia coli. Then Western blotting reactivity of these short peptide fused protein to viral infected sera were surveyed. The results showed that the structural protein linear B-cell epitopes were located on 35 - 81,111 - 161,423 - 453, 462 - 491,531 - 577,616 - 669 and 678 - 732 aa.
作者 于天飞 仇铮 马波 李俚 王君伟
机构地区 东北农业大学动物医学院 齐齐哈尔大学生命科学与工程学院
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第7期96-100,共5页 Biotechnology Bulletin
基金 黑龙江"十五"攻关项目(GB01B503202)
关键词 鹅细小病毒 结构蛋白 线性抗原表位 Goose parvovirus Structural protein Linear epitopes
分类号 S852.65 [农业科学—基础兽医学]
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参考文献11

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二级参考文献19

共引文献59

同被引文献13

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生物技术通报
2010年 第7期

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