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ELISA检测人降钙素基因相关肽在转基因马铃薯块茎中的专一性表达 被引量：1

Tuber-specific Expression of Human Calcitonin-gene Related Peptide in Transgenic Potato by ELISA
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摘要 通过间接ELISA检测了人CGRP基因在转基因马铃薯中的表达。马铃薯class I patatin基因5'侧翼区驱动下人CGRP的表达表现了明显的块茎专一性,茎段中没有检测到CGRP活性,CGRP的表达量在不同转基因株系中有差异,最高达到约50ng/mg可溶性蛋白。 Human calcitonin-gene related peptide（CGRP） expression was detected in transgenic potato by indirect ELISA in this study. The human CGRP expression pattern was tuber-specific directed by 5 ＇flanking region of potato class I patatin gene, but no aetivity was detected in stems. Differences were observed in trausgenic lines with highest of about 50 ng/mg soluble protein. This work lays foundation for neuronal pcptide production by transgenic plant bioreactors.
作者 宋东光 邓日烈 聂呈荣 张英慧 陈淑珍 仇月明 汪训明
机构地区 佛山科学技术学院园艺系 复旦大学遗传所
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第7期117-119,共3页 Biotechnology Bulletin
基金 广东省自然科学基金资助项目(000014)
关键词 人CGRP ELISA 转基因马铃薯 Human CGRP ELISA Transgenic potato
分类号 S532 [农业科学—作物学]
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参考文献10

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二级参考文献56

共引文献20

同被引文献27

  • 1宋艳茹,李枞,侯林林,张莉枝,马庆虎,彭学贤,崔晓江,周雪荣,王海云,莽克强.双价外壳蛋白基因植物表达载体的构建及马铃薯转基因植株的鉴定[J].Acta Botanica Sinica,1994,36(11):842-848. 被引量：21
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生物技术通报
2010年 第7期

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