冬虫夏草培养子实体ITS,5.8S的分析及系统发育研究

ITS and 5. 8S Analysis and Phylogenetic Relationship of Culture Fruiting Body of Cordyceps sinensis( Berk. ) Sacc.

对野生冬虫夏草的人工培养子实体的ITS1,ITS2和5.8S间区序列进行了克隆,并结合已有的序列对ITS1,ITS2和5.8S进行了系统发育分析。结果表明,人工培养子实体与中华被毛孢(Hirsutella sinensis L95DBM-1)具有96%的同源性,在NJ邻接树上形成明显的一支,与虫草属其它类群种间具有明显差异,表明分离培养的虫草子实体为中华被毛孢。遗传距离分析结果显示,培养虫草序列与已知虫草在种内也存在一定差异,可能暗示虫草种群在不同区域具有一定的遗传分化。

The ITS-5.8S sequences were cloned on the culture fruiting body of Cordyceps sinensis. Based on some known ITS- 5.8S sequences in Cordyceps genus,the molecular phylogenetics relationship was performed. The results showed that the ITS-5.8S sequence of the culture asexual fruiting body had 96% identity to that of Hirsutella sinensis（ L95DBM-1 ） and clustered on the obvious lineage. The sequence of culture asexual was different from that of the other species of Cordyceps genus in ITS-5.8S Neighbor-Join phylogenetics tree. This showed that the culture asexual fruiting body was the Hirsutella sinensis. Divergence distance analysis showed that the ITS-5.8S sequence of the culture asexual fruiting body also had great difference in inter-species of C. sinensis,which may suggest that C. sinensis population have certain genetic divergence in different region.