高钾植物DNA导入栽培烟草引起后代性状变异研究

Studies on Variation in Characters of Progeny Obtained by Introducing Total DNA of High Potassium Plants to Tobacco

利用花粉管通道法将高钾植物空心莲子草、商陆和马齿苋的总体DNA导入栽培烟草品种K326中,以引起后代烟叶含钾量大幅度提高。结果表明:3种供体植物DNA导入烤烟后,D0代15%的烟株烟叶钾含量高于2.5%,其中有1株烟叶钾含量高达5.06%。导入后的D9代6个株系的主要农艺性状与受体(K326)相比,株高、叶数、节距和生育期均无多大变化,而在叶面积(长×宽)、茎围和产量3个性状方面与受体相比有较大变化。6个新品系的烟叶含钾量均比对照(K326)有较大的提高,除CD9-08的差异达显著水平外,其它5个新品系均达极显著水平。高钾植物DNA导入栽培烟草,显著改变了受体群体烟叶钾含量,证明导入是有效的。

In order to greatly improve content of potassium in tobacco leaves, total DNA of high potassium plants, alternanthera philoxeroides, pokeweed and purslane, was introduced into tobacco variety K326 by pollen tube pathway. The results showed that the content of potassium of 15% Do generation tobacco plants was over 2.5% and that of one plant reached to 5.06% after DNA of three donors transferred into tobacco. Compared with receptor K326, plant height, leaf numbers, node distance and growth duration of 6 lines of D9 generation tobacco changed unconspicuously, while leaf area, stem thickness and yield changed evidently. The content of potassium of 6 new lines was greatly improved, and CD9-08 and others 5 lines researched significant difference at 0.05 and at 0. 01 probability levels respectively. These results showed that introduction of total DNA of high potassium plants into tobacco to improve potassium content was effective.