摘要
目的和方法：本文利用原位杂交和点杂交的方法，对ＴｒｋＣｍＲＮＡ在大鼠脊髓组织中的分布与坐骨神经损伤后的表达变化进行了研究。结果：ＴｒｋＣｍＲＮＡ几乎存在于所有脊髓神经元与胶质细胞中，在坐骨神经损伤后１ｄ表达增加，以后降到较低水平，到１４ｄ又再次升高，但第二峰较第一峰为低。ＴｒｋＣｍＲＮＡ在大鼠脊髓组织中的广泛分布，表明ＮＴ３对脊髓组织各类神经元均有可能的神经营养作用。坐骨神经损伤后１ｄ表达的增加，可能与损伤应激相关，１４ｄ的再次升高可能由于再生神经渐达靶组织所致。结论：受体表达的改变，可能是受损神经元在损伤与再生修复过程中。
Aim and Methods: NT3 is an
important member of NGF family and performs functions through TrkC and p75 receptors. In situ
hybridization and dot blotting were used to study distribution and cellular localization of TrkC
mRNA in rat spinal cords and its changes following crushing sciatic nerve.Results: The
expression of TrkC mRNA in nearly all neurons suggested widely neurotrophic functions of NT3
for the cells. Dot blotting shows TrkC mRNA increased 1 day after axotomy, went down 3 days
and remained at low level up to 10 days, rose again at 14 days, but second peak was lower
than first peak. The upregulation of TrkC mRNA 1 day after axotomy may be resulted as stress
factor, the second upregulation 14 days associated with rearrangement of regenerating nerve
into target tissues.Conclusion: The changes of TrkC mRNA may be due to conversion of
metabolic status of axotomized of neurons from transmitting mode to growth mode.
出处
《中国应用生理学杂志》
CAS
CSCD
1999年第1期39-42,共4页
Chinese Journal of Applied Physiology
