乳腺癌中Abi1和c-Abl及WAVE 2的表达及其相互关系

EXPRESSIONS OF ABI1,c-ABL AND WAVE 2 PROTEIN IN BREAST CANCER AND THEIR CORRELATIONS

目的研究乳腺癌组织中Abi1、c-Abl和WAVE2蛋白的表达及其相互关系。方法应用免疫组化SP法检测66例乳腺癌组织和24例正常乳腺组织中Abi1、c-Abl和WAVE2蛋白的表达情况。结果 1.正常乳腺组织与乳腺癌组织相比,Abi1和WAVE2蛋白表达有显著性差异(P<0.05),而c-Abl蛋白表达无显著性差异(P>0.05),但有定位的改变。2.Abi1强阳性率与乳腺癌的肿瘤大小、组织学分级、淋巴结转移及临床分期呈负相关性(P<0.05);与患者年龄无关(P>0.05)。c-Abl阳性率及WAVE2强阳性率均与乳腺癌的组织学分级、淋巴结转移及临床分期呈负相关性(P<0.05);与患者年龄及肿瘤大小无关(P>0.05)。3.Abi1蛋白表达与c-Abl和WAVE2蛋白表达呈正相关(P<0.05)。结论乳腺癌中Abi1低表达与预后不良有关。乳腺癌中Abi1蛋白表达的变化可影响c-Abl蛋白的定位和WAVE2蛋白的表达。推测Abi1在Abl/Abi1/WAVE2通路中具有至关重要的地位。

Objective To investigate the expressions of Abi1, c-Abl and WAVE2 proteins in breast cancer and their correlations.Methods The expressions of Abi1, c-Abl and WAVE2 were examined in 66 breast cancer specimens and 24 normal mammary tissues by immunohistochemical staining. Results 1. The strongly positive rates of Abi1 and WAVE2 were significantly lower in breast cancer than in normal controls（P0.05）. The positive rate of c-Abl was not significantly lower in breast cancer than in normal controls（P0.05）, but its location changed. 2. The strongly positive rate of Abi1 was negatively correlated with tumor size, histological grade, lymph node metastasis and clinical stage（P0.05）, but not with age （P0.05）.The positive rate of c-Abl and the strongly positive rate of WAVE2 were negatively correlated with histological grade, lymph node metastasis and clinical stage （P0.05）, but not with age and tumor size （P0.05）. 3.A positive correlation was found between the expression of Abi1 protein and that of c-Abl protein, and between the expression of Abi1 protein and that of WAVE2 protein （P0.05）.Conclusion The reduction of Abi1 expression in breast cancer is correlated with poor prognosis of breast cancer patients. The reduction of Abi1 expression may influence the location of c-Abl protein and the expression of WAVE2 protein. So it is hypothesized that Abi1 may have a key role in the Abl/Abi1/WAVE2 passway.