杏愈伤组织的超低温保存

CRYOPRESERVATION OF APRICOT CALLI

杏茎段在附加１．０ｍｇ／Ｌ２，４－Ｄ和０．１ｍｇ／ＬＢＡ的改良ＭＳ培养基中诱导出愈伤组织，放入１０％ＤＭＳＯ＋０．５ｍｏｌ／Ｌ山梨醇或葡萄糖的冰冻保护剂中，以１℃／ｍｉｎ的降温速度降至－４０℃，停留２ｈ后投入液氮保存，在４０℃水浴中化冻。继代培养８次生长１５－２０ｄ的愈伤组织保存后的相对存活率最高。不同品种基因型保存效果差异很大。保存后的愈伤组织生长量比对照减少。

Apricot ( Armeniaca vulgaris Lam.) calli were initiated from shoot on modified MS medium with 1.0 mg/L 2,4 D and 0.1 mg/L BA,put into 10% DMSO+0.5% mol/L sorbitol or glucose,cooled at a rate of 1℃/min from 0℃ to -40℃,kept -40℃ for 2 hours,put into liquid nitrogen and then thawed in 40℃ water bath.Calli which have been subcultured 8 times and grown 15-20 days showed highest survival rate.There was significant difference in survival rate among three cultivars tested.The growth of the calli after cryopreservation were retarded,but after subcultured 2 times,no difference was found between the treatment and control.