栗的 PGI遗传和多样性(英文)

INHERITANCE AND DIVERSITY OF PGI IN CHESTNUT (CASTANEA)

采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法 ,分析了栗属种的 PGI同工酶的遗传。研究发现 Pgi位点 ( Pgi 1 )主要有 3个等位基因并呈共显性遗传。在栗属的自然居群中还检测到了出现频率较少的另外 2个等位基因。美洲栗 ( Castanea dentata)和中国板栗 ( C.mollissima)居群的 Pgi基因频率和遗传杂合度存在较大的差异。中国板栗的 Pgi位点的遗传杂合度通常高于美洲栗。在美洲栗自然分布区中 ,南端地区的居群存在显著高的 Pgi遗传杂合度。在中国板栗居群中 ,长江流域居群 Pgi杂合度最高 ,东南部居群最低。

Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method.Three alleles at one Pgi locus (Pgi 1) were found to be codominantly inherited.Two additional alleles were detected in samples of natural populations.Considerable variations in allele frequency and heterozygosity were found in populations of the American (Castanea dentata) and Chinese (C. mollissima) chestnut species.Heterozygosity at the Pgi locus was generally higher in Chinese than in American chestnut.Significantly higher heterozygosity of the Pgi was detected in the southernmost location of the natural range of the American chestnut.The highest and lowest heterozygosity was observed in Chinese chestnut populations from the Changjiang River and southeast region of China, respectively.