摘要
目的：建立以高效液相色谱法测定谷维素双维B片中维生素B6含量的方法。方法：以Ultimatec18柱（250mm×4．6mm，5μm）为色谱柱，甲醇O．075mol／L磷酸二氢钾溶液（13：87）为流动相，流速为1．0mL／min，检测波长为291nm。结果：维生素B6浓度在40-200μg／mL范围内与峰面积线性关系良好（r=0．9999，n=7），平均回收率为98．89％，RSD=0．60％（n=6）。结论：该方法简便、快速、准确可靠，可用于谷维素双维B片中维生素B6的含量测定。
Objective To establish a HPLC method for the determination of Vitamin B6 in Oryzaol. Vitamin B1 and Vitalmin B6 Tabietso Method Chromatographic condition included Ultimate C18 （ 250mm×4.6mm,5μm） column and mobile phase of mixture of methanol-0.075mol/L potassium dihydrogen phosphate （ 13： 87） o Flow rate was 1.0mL/min, the detection wavelength was 291nmo Result The standard curve of Vitalmin B6 was linear in the concentration from 40-200 μ g/mL （r=0.999 9, n=7） , the average recovery rate was 98.89%, RSD=0.60%（n=6）. Conclusion This method is simple, rapid, accurate and can be used for the determination of Vitamin B6 in Oryzaol, Vitamin B1 and Vitalmin B6 Tablets
