碱液提取箬叶多糖的纯化及其结构性质的研究

Study on the Isolation, Purification and Structural Property of Polysacchrides from Ruoye ( Indocalamus tesselatus).

通过高碘酸氧化、Ｓｍｉｔｈ降解、部分酸水解分析、ＮＭＲ分析等多种方法对以不同浓度的ＮａＯＨ溶液箬叶中提取的两种多糖ＦⅢａ及ＦⅣａ进行了研究，结果表明二者均具有多分枝结构，ＦⅢａ主链以α（１→３）连接的木糖为主，分子侧链由半乳糖、阿拉伯糖、葡萄糖醛酸构成，葡萄糖醛酸主要位于分子的末端；ＦⅣａ主链由α（１→３）木糖和β（１→６）半乳糖构成，以阿拉伯糖、葡萄糖醛酸组成侧链，葡萄糖醛酸主要位于分子的末端．

Two polysaccharides which were prepared from Chinese traditional medicine Ruoye ( Indocalamus tesselatus ), a kind of bamboo leaves by alkaline extraction were studied. Their physicochemical properties and structural feature were elucidated by modern analytical technique such as IR, NMR, GC, partial acid hydrolysis, periodate oxidation and Smith degradation. The typical absorptions of these polysaccharides were shown in IR spectra. The main structure was examined: FⅢ a, (1→6) β D galactosyl branched (1→3) α D Xylan and FⅣ a, (1→6) β D galactosyl (1→3) α D Xylan with arabinose branches at C 6 position.