摘要
对检测人结肠癌组织端粒酶活性的端粒酶重复序列扩增法（ＴＲＡＰ）进行了研究．当Ｍｇ２＋浓度为１８ｍｍｏｌ／Ｌ、退火温度为５５℃时，能得到可靠的检测结果．影响检测结果可靠性的另两个因素是抽提物的反应用量和阳性片段的污染．
A TRAP assay on human colonic tumor telomerase activity is reported. The reliable result is obtained when 1 8 mmol/L Mg 2+ and 55℃ annealing temperature are adopted. The other two factors affecting reliability are quantity of the extract and contamination of the positive DNA fragments in the reaction.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第1期72-75,共4页
Progress In Biochemistry and Biophysics
基金
上海市自然科学基金
关键词
结肠癌组织
端粒酶活性
重复序列扩增法
human colonic tumor tissue, telomerase activity, telomeric repeat amplification protocol
