胡萝卜细胞培养产胡萝卜素的研究

Studies on the formation of β-carotene in cell suspension cultures of Daucus carota

从鲜红胡萝卜主根部选取外植体，用MS型培养基诱导出愈伤组织，继代培养，周期22d，接着进行细胞的液体悬浮培养。液体培养20d时，测定β-胡萝卜素含量达41．7mg／L，占细胞干重的0．35％。培养细胞中含胡萝卜素是胡萝卜根部的3．38倍，色素比产率提高15．4倍。

The callus was derived from the explants, selected from the main root of Daucus carota,in MS medium. One growth period was about 22 days for proliferation, and then callus was subcultured into suspension culture. The optimum period of harvesting in cell suspension culture was 20 days. The result showed that the content of β-carotene can reach 41.7 mg/L and is 0.35 %of dry cell weight. The pigment content of moist cell in suspension culture is 3.38 times of that of main root of Daucus carota and β-carotene yield increase 15.4 fold.