摘要
研究了从桑叶中提取分离黄酮的方法,探讨了提取剂、料液比、回流时间、回流温度等因素对桑叶黄酮提取率的影响,并通过正交实验对提取工艺进行了优化。实验表明,提取最佳条件为:将原料用超声波预处理40 m in(800 W),以80%的乙醇为提取剂,按料液比1∶8提取,回流温度为70℃,AB-8大孔吸附树脂分离,以乙醇洗脱(洗脱速率为1 BV/h),得率为19.3 mg/g,同时测得黄酮的稳定性良好。本方法具有工艺流程简单、提取率高的优点,适合于工业化生产,为同类研究提供了一定的依据。
The method extracting flavonoids from mulberry leaf was researched.The effect of extracting agent,liquid ratio,reflux time,temperature and other factors on the yield of flavonoids were studied,and the extraction process were optimized by orthogonal experiment.Results showed that： the best conditions for the extraction of raw materials were using ultrasonic pre-treatment 40 min（800 W）,80% ethanol as the extraction agent,the ratio of material to liquid was 1∶8,the reflux temperature was 70 ℃,using AB-8 macroporous adsorption resin and ethanol elution（elution rate 1 BV/h）,the yield was 19.3 mg/g,and the stability of flavonoids was good.This method has some advantages,such as simple technological process,high extraction rate,and is suitable for industrial production,meanwhile,it provides some data for the same research.
出处
《应用化工》
CAS
CSCD
2010年第6期790-792,795,共4页
Applied Chemical Industry
基金
国家973项目(G1999403606)
关键词
桑叶
黄酮
回流
提取率
mulberry leaf
flavonoids
reflux
extraction rate
