摘要
目的:探讨苦参总生物碱、总黄酮提取物及其混合物的体外抗菌效果。方法:采用液体二倍稀释法分析苦参总生物碱、苦参总黄酮提取物及其混合物对金黄色葡萄球菌、大肠埃希菌、福氏志贺菌、乙型副伤寒杆菌、铜绿假单胞菌、白色葡萄球菌、痢疾杆菌和柠檬色葡萄球菌的抗菌作用,并测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:苦参总生物碱对以上菌株的MIC分别为:9.00、3.38、8.25、9.00、4.50、13.50、6.75、7.13mg.mL-1,MBC分别为:13.50、14.25、8.25、9.00、14.25、14.25、13.13、14.25mg.mL-1;苦参总黄酮对以上菌株的MIC分别为3.00、2.63、3.56、3.38、1.50、2.44、3.56、2.35mg.mL-1,MBC分别为14.25、12.75、7.13、13.50、13.50、12.75、12.75、13.13mg.mL-1;苦参总生物碱与总黄酮混合物对以上菌株的MIC分别为:2.25、2.07、3.28、3.94、1.22、2.44、2.07、1.97mg.mL-1,MBC分别为9.00、13.13、6.75、6.38、13.88、7.50、6.38、7.13mg.mL-1。结论:苦参总生物碱和总黄酮提取物对上述菌株存在体外抑菌和杀菌活性,混合物呈现一定协同作用。
Objective:Discuss the antibacterial effects of alkaloids,flavonoids which extract from Sophora flavescens Ait.and their mixture in vitro.Methods: Using doubling dilution methods to analyze the antibacterial effect of alkaloids,flavonoids which extract from Sophora flavescens Ait.and their mixture to Pseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus,Shigella,Shigella flexneri,Staphylococcus citreus,Staphylococcus albus,and Bacillus paratyphosus B in vitro,to determine the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC).Results: MIC of the above strains,alkaloids were: 9.00,3.38,8.25,9.00,4.50,13.50,6.75,7.13mgomL-1,flavonoids were: 3.00,2.63,3.56,3.38,1.50,2.44,3.56,2.35mgomL-1,the mixture were2.25,2.07,3.28,3.94,1.22,2.44,2.07,1.97mgomL-1;MBC of the above strains,alkaloids were: 13.50,14.25,8.25,9.00,14.25,14.25,13.13,14.25mgomL-1,flavonoids were: 14.25,12.75,7.13,13.50,13.50,12.75,12.75,13.13mgomL-1;the mixture were: 9.00,13.13,6.75,6.38,13.88,7.50,6.38,7.13mg o mL-1.Conclusion: Alkaloids,flavonoids which extract from Sophora flavescens Ait.had antibacterial and bactericidal activity in vitro,the mixture had synergy.
出处
《中医药学报》
CAS
2010年第3期74-76,共3页
Acta Chinese Medicine and Pharmacology
基金
国家教育部春晖计划(编号:Z2008-1-15016)
关键词
苦参提取物
总生物碱
总黄酮
体外抗菌实验
Extracts of Sophora flavescens Ait.
Alkaloids
Flavonoids
Antibacterial activity in vitro