络合协同RDB处理NO_x系统中1株反硝化菌的分离鉴定

Identification and Phylogenetic Analysis of an Isolated Denitrifier from Rotating Drum Biofilter

从处理效率稳定在85%的NOx废气络合处理系统--生物转鼓(rotating drum biofilter,RDB)中分离到1株异养型兼氧反硝化细菌,命名为菌株ND1.理化分析表明,ND1为革兰氏染色阴性杆菌,在培养基上可形成特征性干燥、皱缩样菌落,黏附于琼脂表面,并产生黄色色素,以单极生鞭毛运动.其16S rDNA基因序列与典型的反硝化菌Pseudomonas stutzeri具有97%的相似性,综合其外部形态特征、生理生化特征、Biolog碳源利用特性以及16S rDNA系统发育学分析,ND1鉴定为Pseudomonasstutzeri.在30℃,pH7.2的培养条件下,以琥珀酸钠为碳源,5d后该菌对硝酸根离子的去除率可以达到100%,对相同浓度的亚硝酸根离子的去除率达到85%.

A heterotrophic denitrifying strain named ND1 has been isolated from rotating drum biofilter（RDB） which was mainly used for the treatment of exhaust gas such as NOx.It was proved to be Gram-negative.In addition,it was able to form dry and wrinkled colonies on the medium,adhere on the agar surface,produce yellow pigments and be motile with a single polar flagellum.It was indentified as the Pseudomonas stutzeri according to morphological and physiological biochemical characteristics and 16S rDNA sequence analysis.Under the conditions of pH 7.2 and temperature 30℃,the NO 3--N removal efficiency by ND1 was up to 100% in 5 days,while the NO 2--N degradation ratio was only 85% by the strain DN1 when subjected to the same condition as the above.