吡啶降解菌中质粒的功能探讨

Study on the Function of Plasmid in Pyridine-Degrading Bacteria

为了探讨吡啶降解菌质粒的特性及其与降解的关系,对降解吡啶的2株细菌Paracoccus sp.BW001及Shinellazoogloeoides BC026进行了质粒提取和脉冲电泳实验,确定BW001含有2个190～245kb的大质粒和1个4.5～5.0kb的小质粒,而BC026含有至少3个超过200kb的大质粒.通过高温-SDS法对含有质粒的2株菌进行质粒消除实验,发现质粒消除后的细菌不再降解吡啶,推测降解吡啶的基因可能存在于质粒上.通过电转化将Paracoccus sp.BW001的质粒转入E.coli5α中,发现转化后的菌株具有耐受吡啶的特性.

In order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids＇ function and biodegradation,plasmids were isolated from two bacterial strains （Paracoccus sp.BW001 and Shinella zoogloeoides BC026） and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size.Two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the BW001,and at least 3 large plasmids over 200 kb were harbored in the BC026.The plasmid curing was conducted by high temperature-SDS method and the results indicated the biodegradation genes might locate in the plasmids of two bacterial strains.After transforming the plasmids of BW001 into E.coli 5α by electroporation,the new bacterial strain could tolerate pyridine.