子宫内膜异位症患者腹腔液及血清中IL-12的水平测定

Determination of interleukin-12 level in peritoneal fluid and serum of patients with endometriosis

目的:探讨子宫内膜异位症(EM)患者腹腔液及血清中白细胞介素-12(IL-12)水平的变化及意义。方法:采用ELISA法检测39例EM患者及16例非EM的对照者腹腔液及血清中IL-12水平。结果:EM组腹腔液及血清中IL-12水平均低于对照组(P<0.01)。EM组Ⅲ-Ⅳ期腹腔液IL-12[(62.37±13.7)ng/L]和血清IL-12[(53.64±17.4)ng/L]均低于Ⅰ-Ⅱ期患者腹腔液[(76.38±13.5)ng/L]和血清[(75.67±12.3)ng/L]水平(P<0.01)。结论:IL-12在EM发病中起重要作用,检测其含量变化对于该病的诊断治疗具有参考意义。

Objective：To explore the changes and clinical significance of interleukin-12（IL-12）in the peritoneal fluid and serum of patients with endometriosis.Methods：IL-12 in the peritoneal fluid and serum of 39 patients with endometriosis and 16 controls were detected by enzyme linked immunosorbent assay（ELISA）.Results：IL-12 levels in the peritoneal fluid and serum of the endometriosis group were lower than those of the control group（P0.01）.IL-12 levels in the peritoneal fluid [（62.37±13.7）ng/L] and in the serum [（53.64±17.4）ng/L] of patients with stageⅢ-Ⅳ endometriosis were lower than those in the peritoneal fluid[（76.38±13.5）ng/L]and in the serum [（75.67±12.3）ng/L] of patients with stageⅠ-Ⅱendometriosis（P0.01）.Conclusions：IL-12 may play an important role in the pathogenesis of endometriosis.Determining the IL-12 levels in patients with endometriosis could help to the diagnosis and treatment.