四川南江两种水青冈种群遗传多样性初步研究

STUDIES ON GENETIC DIVERSITY OF CHINESE BEECHES IN NANJIANG,SICHUAN PROVINCE

利用凝胶电泳法研究米心水青冈（Ｆａｇｕｓｅｎｇｌｅｒｉａｎａ）和巴山水青冈（Ｆ．ｐａｓｈａｎｉｃａ）２个种４个种群的遗传多样性。所测定的酶系统包括：过氧化物酶（ＰＸ１和ＰＸ２），磷酸葡萄糖脱氢酶（ＰＧＤ），酸性磷酸化酶（ＡＣＰ），超氧物歧化酶（ＳＯＤ），谷氨酸草酰乙酸转氨酶（ＧＯＴ１和ＧＯＴ２），异柠檬酸脱氢酶（ＩＤＨ），磷酸果糖异构酶（ＰＧＩ），甲基萘醌还原酶（ＭＮＲ），葡萄糖磷酸变位酶（ＰＧＭ１和ＰＧＭ２）和苹果酸脱氢酶（ＭＤＨ２）１０种酶系统。测定和分析了水青冈等位基因频率、遗传多样性、固定指数、Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡和遗传距离指标。

A gel electrophoresis method,was used to study the genetic diversity of Fagus engleriana and F.pashanica at Nanjiang Country,Sichuan Province.Ten allozymes were identified and they are:Peroxydase(PX 1 and PX 2),Phosphogluconate dehydrogennase(PGD),Acide phosphatase(ACP),Superoxyde dismutase(SOD),Glutamate oxaloacetate transaminase(GOT 1 and GOT 2),Isocitrate dehydrogenase(IDH),Phosphate glucose isomerase(PGI),Menadione reductase(MNR),Phosphoglucomutase(PGM 1 and PGM 2),and Malate dehydrogenase(MDH 2).We have determined and analysed the allelic frequency,genetic diversity,fixation index,Hardy Weinberg equilibrium,and genetic distances of the 2 beech species.This results could be applied in the silvicultural practice,such as genetic improvmemt and species selection,and they could also provide some basic scientific information for the further studies of taxonomic relations and systematic evolution of the beech species in China.