旋转脉动磁场对兔牙移动过程中牙周组织内血小板衍生生长因子A表达的影响

A study on expression of platelet derived growth factor a in periodontal tissue during orthodontic tooth movement with rotating pulsed magnetic field

目的 探讨旋转脉动磁场对兔实验性牙齿移动过程中牙周组织血小板衍生生长因子A（PDGF-A）表达的影响.方法 30只体重为1.0～1.5 kg的健康新西兰大耳白兔按每组5只随机分为实验1、3、5、7、14、21 d组.2%戊巴比妥钠麻醉下于双侧上颌第一磨牙与切牙间拴结不锈钢螺簧,施力80g,左侧只加力为对照侧,右侧加力加旋转脉动磁场为实验侧,通过免疫组织化学染色进行PDGF-A半定量分析.结果 实验侧牙周组织中PDGF-A表达高于对照侧;实验侧张力区、压力区牙周组织中PDGF-A表达在5、7、14 d时较对照侧差异有统计学意义（5.28±0.14 vs 2.03±0.18,7.63±0.27 vs 2.84±0.12,3.52±0.16 vs 1.65±0.03;8.10±0.13 vs 4.30±0.21,13.27±0.31 vs 6.47±0.15,5.66±0.22 vs 3.15±0.27,P＜0.05）;在7 d时牙周组织PDGF-A表达达到高峰.结论 旋转脉动磁场照射促进了牙周组织中PDGF-A的表达,促进了牙周组织的改建.

Objective To investigate the effects of rotating pulsed magnetic field on platelet derived growth factor a （PDGF-A） expression in periodontal tissue during tooth movement. Methods 30 white rabbits were random divided into 6 groups with 5 rabbits each group, including groups of 1,3,5,7,14 and 21 days. Under anesthesia condition by 2% pentobarbital sodium, the stainless coil springs were fixed between the first maxillary molar and the incisor producing the force of 80g. The experimental group was treated by rotating pulsed magnetic field and the force, the control group was only treated by the force. The expression of PDGF-A was half-quantitatively investigated through immunohistochemieal analysis. Results The expression of PDGF-AA in the experimental group enhanced apparently compared with that in the control group. There were significant differences among the 5,7, and 14day groups （ 5.28 ± 0. 14 vs 2.03 ± 0. 18,7. 63 ± 0. 27 vs 2. 84 ± 0. 12,3.52 ± 0. 16 vs 1.65 ± 0. 03 ;8.10 ± 0. 13 vs 4. 30 ± 0. 21,13.27 ± 0. 31 vs 6. 47 ±0. 15,5.66 ±0. 22 vs 3.15 ±0. 27, P 〈0. 05）. The expression of PDGF-AA of 7 day group was higher than that of other groups. Conclusion Rotating pulsed magnetic field promotes the expression of PDGF-A in the periodontal tissue and alveolar bone remodeling.