慢性乙型肝炎患者血清XCL1与免疫损伤的相关性分析

Analyze the relationship between serum XCL1 levels and the immune injury in CHB patients

目的 探讨慢性乙型肝炎患者血清XCL1含量与肝损程度及HBV DNA含量的相关性,探讨XCL1表达变化的可能原因.方法 采用酶联免疫吸附法（ELISA）检测血清XCL1浓度;流式细胞仪检测T淋巴细胞亚群;时间分辨荧光分析仪检测乙型肝炎抗原/抗体半定量指标;全自动荧光定量PCR仪检测HBV-DNA载量;全自动生化仪检测肝功能.结果 （1）正常对照组、慢性乙肝（轻度）组、慢性乙肝（中度）组、慢性乙肝（重度）组血清XCL1浓度分别为（8.24±1.94）pg/ml、（10.99±1.94）pg/ml、（12.83±2.59）pg/ml、（13.72±3.13）pg/ml,慢性乙肝（轻、中、重度）组血清XCL1浓度显著高于对照组（P＜0.05）;慢性乙肝（重度）组血清XCL1浓度明显高于慢性乙肝（轻度）组（P＜0.05）;慢性乙肝（中度）组与慢性乙肝（轻、重）度组血清XCL1浓度比较,差异无统计学意义（P＞0.05）;XCL1浓度与ALT、AST、TBIL、DBIL水平呈正相关（r=0.463、0.472、0.413、0.440,P＜0.01）.（2）乙肝病毒低载量组血清XCL1浓度为（11.43±2.01）pg/ml,与高载量组相比差异有统计学意义（P＜0.05）,乙肝病毒低载量组及高载量组血清CD4＋T细胞百分比分别为（41.26±11.33）%、（33.01±5.96）%,两者比较差异有统计学意义（P＜0.05）.结论 XCL1水平与肝脏炎症程度密切相关,可反映HBV感染后机体的免疫状态.

Objective To explore the relevance between serum XCL1 levels and liver damage in hepatitis B patients. Methods The serum concentration of XCL1 was detected by enzyme linked immunosorbent assay （ELISA）. Peripheral blood T-cell subsets were detected by flow cytometry （FCM）. Liver function was assayed by automatic biochemistry analyzer, hepatitis B antigen/antibody semi-quantitative index was detected by time-resolved fluorescence analyzer, and HBV-DNA load was detected by automatic fluorescence quantitative PCR. Results Serum concentration of XCL1 in control group （ n = 20） , mild chronic hepatitis B （CHB） group （ n =29）, moderate CHB group （ n =20） and severe CHB group （ n =26） were （8.24±1.94） pg/ml, （10.99±1.94） pg/ml, （12.83±2.59） pg/ml, （13.72 ±3. 13） pg/ml, respectively. The ＇concentration of XCL1 in all CHB groups was significantly higher than control group （ P 〈 0. 05 ）. The concentration of XCL1 in severe CHB group was significantly higher than mild CHB group （ P 〈 0. 05）. XCL1 was positively correlated with ALT, AST, TBIL and DBIL, and the coefficients were （ r =0. 463, 0. 472, 0. 413, 0. 440, P 〈0. 01 ）, respectively. The serum XCL1 levels in hepatitis B virus with low load group was lower than hepatitis B virus with high load group. The percentage of CD4^＋ T in hepatitis B virus with low load group and high load group were （41.26 ± 11.33）%, （33.01 ±5.96）%, and the difference was statistically significant. Conclusion Serum concentrations of XCL1 were closely related to the degree of liver inflammation in hepatitis B patients. XCL1 may be involved in the process of chronic hepatitis B.