hTERT CD44v6在大肠癌中的表达及意义

The Expression and Significance of hTERT and CD44v6 in Colorectal Carcinoma

目的:探讨hTERT、CD44v6的表达与大肠癌发生、浸润转移及预后的关系。方法:对大肠腺瘤组织及大肠癌组织进行免疫组织化学染色,分析hTERT及CD44v6在大肠癌中表达的相关性。结果:hTERT及CD44v6在大肠癌组织中的阳性表达率分别为70.73%、53.66%,明显高于大肠腺瘤组织中的10.53%、10.53%(P<0.01);hTERT及CD44v6在有淋巴结转移的大肠癌患者的阳性表达率分别为93.33%、86.67%,明显高于无淋巴转移患者的阳性表达率53.85%、38.46%(P<0.01);hTERT及CD44v6在TNMⅢ、Ⅳ期大肠癌患者的阳性表达率分别为94.12%、82.35%,明显高于TNMⅠ、Ⅱ期的54.17%、33_33%(P<0.01);在生存期<5年大肠癌中的阳性表达率分别为90.91%、77.27%,明显高于生存期≥5年的47.37%、26.32%(P<0.01);hTERT和CD44v6在性别、年龄、肿物大小、肿物位置及浸润深度中的阳性表达差异均无统计学意义(P>0.05)。结论:hTERT和CD44v6在大肠癌组织中具有高表达,特别是在有淋巴结转移、TNMⅢ、Ⅳ期和生存期<5年组中有较高表达,说明两者可能在大肠癌的发生、浸润转移过程中起着重要作用,并且与大肠癌患者的预后有关;联合检测hTERT和CD44v6可以作为大肠癌浸润转移的判断和预后评估的参考指标。

Objective： To explore the relationship of hTERT and CD44v6 expression with the carcinogenesis, infiltration, metastasis, and prognosis of colorectal carcinoma. Methods： Immunohistochemistry was used to detect the expression of hTERT and CD44v6 in the tissue of colorectal carcinoma and adenoma. Results： The positive rates of expression of hTERT and CD44v6 in colorectal carcinoma tissue were 70.73% and 10.53%, respectively; the positive rates of expression of hTERT and CD44v6 were 10.53% and 10.53% in colorectal adenoma tissue, respectively, with a significant difference between the 2 groups （P〈0.01）. The positive rates of expression of hTERT and CD44v6 in colorectal carcinoma tissue from patients with lymph node metastasis were respectively 93.33% and 86.67%, distinctly higher than those from patients without lymph node metastasis （53.85% and 38.46%）, with a significant difference （P〈0.01）. The positive rates of hTERT and CD44v6 expression in III and IV stage colorectal carcinoma tissue were 94.12% and 82.35%, higher than those in TNM I and II stage colorectal carcinoma tissue （54.17% and 33.33%）, with a significant difference （P〈 0.01）. The positive rates of hTERT and CD44v6 expression in colorectal carcinoma patients with survival of less than 5 years were 90.91% and 77.27%, distinctly higher than those in patients with survival of 5 or more years （P〈0.01）. Expression of hTERT and CD44v6 was not correlated with sex, age, tumor size, tumor location, or depth of infiltration （/：＇〉0.05）. Conclusion： High expressions of hTERT and CD44v6 exist in colorectal carcinoma, especially in the tissue from patients with lymph node metastasis, TNM stages III and IV, and survival of fewer than 5 years, hTERT and CD44v6 probably play an important role in the carcinogenesis, invasion, and metastasis of colorectal carcinoma, hTERT and CD44v6 could be an index for the evaluation of infiltration, metastasis, and prognosis of colorectal carcinoma.