计算机电磁辐射对小鼠学习记忆和大脑神经递质的影响

Influence of computer electromagnetic radiation on learning and memory ability as well as cerebra neuron transmitter of mice

为研究模拟网吧环境的计算机电磁辐射(Computer radiation,CR)对小鼠学习记忆及大脑神经递质的影响,采用清洁级雄性昆明小鼠32只,随机分为对照组(Control)、短时组(CR6h/d)、中时组(CR12h/d)和长时组(CR18h/d),各辐照组小鼠放置在模拟网吧环境开机运行的计算机正前方20cm处(电场强度0.9V/m),连续30d辐照,对照组被安排在无辐射区。第31天采用Y型迷宫试验测试小鼠学习记忆能力,并测定小鼠大脑三种神经递质。结果发现,计算机辐射可导致小鼠学习记忆能力下降,大脑组织乙酰胆碱(Ach)含量下降,一氧化氮(NO)含量和谷氨酸(Glu)含量升高,乙酰胆碱脂酶(AchE)和一氧化氮合酶(NOS)活性升高,谷氨酰胺合成酶(GS)活性下降。以上结果说明,计算机电磁辐射降低了小鼠的学习记忆能力,这可能与其影响了小鼠脑部的神经传递递质有关。

In order to observe the effects computer radiation（CR） on learning and memory ability as well as cerebra neuron transmitter of mice, the 32 male Kunming mice were randomly divided into four groups as one control group and three CR groups irradiated for 6 h/d, 12 h/d, and 18 h/d, respectively. The three CR groups were settled 20cm before the operating computer （0.9 V/m） for 30 days consecutively. And the control group was placed in the environment without computer radiation （0 V/m）. The learning and memory ability of the mice were measured by Y-type maze test at thirty-first day, then the activity of acetylcholinesterase （AchE）, nitric oxide synthase （NOS） and glutamine synthetase （GS） were measured as well as the content of acetylcholine（Ach）, nitric oxide（NO） and Gluta- mate （Glu）. The results show that the content of Ach in cerebra decreased, but the content of NO and Glu were just the opposite; the activities of AchE and NOS were enhanced, but the activity of GS was just the opposite. It is re- vealed that the computer radiation can reduce the learning and memory ability of mice, which may involve the changes of cerebra neuron transmitter.